Dr. Puthuff is a native of Louisville, Kentucky. She graduated from the University of Louisville School of Medicine in 1987. Following her internship plus two years of internal medicine residency at the University of Louisville, she completed a dermatology residency at Ohio State University in 1995. She is board certified in dermatology and maintains this status with regular recertification.
She also volunteers at the Family Community Clinic in Louisville, Kentucky. She is a member of the Greater Louisville Medical Society, Kentucky Medical Society, Kentucky Dermatologic Society, and the American Academy of Dermatology. She currently serves on the Greater Louisville Medical Society Foundation board and is active in international and local mission work.